分子生物学教室

研究業績

<2018年>
A negative feedback loop between Nuclear Factor 90 (NF90) and an anti-oncogenic microRNA, miR-7
Takuma Higuchi, Keiko Morisawa, Hiroshi Todaka, Sylvia Lai, Eunsup Chi, Kazutsugu Matsukawa, Yasunori Sugiyama, Shuji Sakamoto
Biochem. Biophys. Res. Commun., doi: 10.1016/j.bbrc.2018.07.119. [Epub ahead of print] (2018 Jul 24)

A method for profiling the phosphorylation state of tyrosine protein kinases
Yuuki Uezato, Isamu Kameshita, Keiko Morisawa, Shuji Sakamoto, Eiji Kinoshita, Emiko Kinoshita-Kikuta, Tohru Koike, Yasunori Sugiyama
Biochim. Biophys. Acta., doi: 10.1016/j.bbapap.2018.05.003. [Epub ahead of print] (2018 May 9)


An NF90/NF110-mediated feedback amplification loop regulates dicer expression and controls ovarian carcinoma progression
Jérôme Barbier, Xin Chen, Gabriel Sanchez, Muyan Cai, Marion Helsmoortel, Takuma Higuchi, Pierre Giraud, Xavier Contreras, Gangjun Yuan, Zihao Feng, Rima Nait-Saidi, Olivier Deas, Lisa Bluy, Jean-Gabriel Judde, Sylvie Rouquier, William Ritchie, Shuji Sakamoto, Dan Xie & Rosemary Kiernan
Cell Res.  doi: 10.1038/s41422-018-0016-8. [Epub ahead of print] (2018 Mar 21)


<2016年>
microRNA 生合成が引き起こす病態生理現象
戸高 寛, 樋口 琢磨, 坂本 修士
比較生理学, Vol. 34, No. 4, 183-190 (2016)

Suppression of miR-7 biogenesis by NF90-NF45 controls cell proliferation in hepatocellular carcinoma
Takuma Higuchi, Hiroshi Todaka, Yasunori Sugiyama, Masafumi Ono, Nobuyuki Tamaki, Etsuro Hatano, Yuka Takezaki, Takeshi Miwa, Sylvia Lai, Keiko Morisawa, Masayuki Tsuda, Taketoshi Taniguchi, Shuji Sakamoto
J. Biol. Chem., 291(40), 21074-21084 (2016)


PNPLA3 is involved in hepatic fatty acid and triglyceride metabolism through XBP1 and modulation of endoplasmic reticulum stress in mice
Tsunehiro Ochi, Kensuke Munekage, Masafumi Ono, Takuma Higuchi, Masayuki Tsuda, Yoshihiro Hayashi, Nobuto Okamoto, Katsumi Toda, Shuji Sakamoto, Jude A Oben, Toshiji Saibara
Hepatology Res., 46(6), 584-592 (2016)

<2015年>
Overexpression of NF90-NF45 represses myogenic microRNA biogenesis, developing skeletal muscle atrophy and centronuclear muscle fibers
Hiroshi Todaka, Takuma Higuchi,
Ken-ichi Yagyu, Yasunori Sugiyama, Fumika Yamaguchi, Keiko Morisawa, Masafumi Ono, Atsuki Fukushima, Masayuki Tsuda, Taketoshi Taniguchi, Shuji Sakamoto
Mol. Cell. Biol., 35(13), 2295-2308 (2015)

Expression and phosphorylation state analysis of intracellular protein kinases using Multi-PK antibody and Phos-tag SDS-PAGE
Yasunori Sugiyama, Syouichi Katayama, Isamu Kameshita, Keiko Morisawa, Takuma, Higuchi, Hiroshi Todaka, Eiji Kinoshita, Emiko Kinoshita-Kikuta, Tohru Koike, Taketoshi Taniguchi, Shuji Sakamoto
MethodsX, 2, 469-474 (2015)

マルチPK抗体とPhos-tagを利用した細胞内リン酸化動態の新規解析法
杉山 康憲, 亀下 勇, 坂本 修士
生物物理化学‐電気泳動‐, 59(2), 82-84 (2015)

<2014年>
Bofutsushosan, a Japanese herbal (Kampo) medicine, attenuates
progression of nonalcoholic steatohepatitis in mice

Masafumi Ono, Mitsunari Ogasawara, Akira Hirose, Sachiko Mogami, Nobuhiro Ootake, Kosuke Aritake, Takuma Higuchi, Nobuto Okamoto, Shuji Sakamoto, Masahiro Yamamoto, Yoshihiro Urade, Toshiji Saibara, Jude A. Oben
J. Gastroenterol., 49(6), 1065-1073 (2014)

<2012年>
High expression of nuclear factor 90 (NF90) leads to mitochondrial degradation in skeletal and cardiac muscles
Takuma Higuchi, Shuji Sakamoto, Yoshihiko Kakinuma, Shoko Kai, Ken-ichi Yagyu, Hiroshi Todaka, Eunsup Chi, Shoshiro Okada, Takako Ujihara, Keiko Morisawa, Masafumi Ono, Yasunori Sugiyama, Waka Ishida, Atsuki Fukushima, Masayuki Tsuda, Yasutoshi Agata, Taketoshi Taniguchi
PLoS One, 7, e43340 (2012)

PKL01, an Ndr kinase homologue in plant, shows tyrosine kinase activity
Syouichi Katayama, Yasunori Sugiyama, Naoya Hatano, Toru Terachi, Noriyuki Sueyoshi, Isamu Kameshita
J. Biochem., 152, 347-353 (2012)

E2A and CBP/p300 Act in Synergy To Promote Chromatin Accessibility of the Immunoglobulin k Locus
Shuji Sakamoto, Kousho Wakae, Yuki Anzai, Kiyohito Murai, Nobuyuki Tamaki, Masaki Miyazaki, Kazuko Miyazaki, William J. Romanow, Tomokatsu Ikawa, Daisuke Kitamura, Itaru Yanagihara, Nagahiro Minato, Cornelis Murre, Yasutoshi Agata
J. Immunol., 188, 5547-5560 (2012)

Highly sensitive detection of protein phosphorylation by using improved Phos-tag Biotin
Eiji Kinoshita, Emiko Kinoshita-Kikuta, Yasunori Sugiyama, Yoshitaka Fukada, Tomomitsu Ozeki, Tohru Koike
Proteomics, 12, 932-937 (2012)

Multiple sites of highly amplified DNA sequences detected by molecular cytogenetic analysis in HS-RMS-2, a new pleomorphic rhabdomyosarcoma cell line
Eiji Takaoka, Hiroshi Sonobe, Kunihiro Akimaru, Shuji Sakamoto, Taro Shuin, Masanori Daibata, Takahiro Taguchi, Akira Tominaga
Am. J. Cancer Res., 2, 141-152 (2012)

<2011年>
Regulation of Experimental Autoimmune Uveoretinitis by Anti-Delta-Like Ligand 4 Monoclonal Antibody
Waka Ishida, Ken Fukuda, Shuji Sakamoto, Noriko Koyama, Akemi Koyanagi, Hideo Yagita, Atsuki Fukushima
Invest. Ophthalmol. Vis. Sci., 52, 8224-8230 (2011)

CoPK32 is a novel stress-responsive protein kinase in the mushroom Coprinopsis cinerea
Keisuke Kaneko, Yasunori Sugiyama, Yusuke Yamada, Noriyuki Sueyoshi, Akira Watanabe, Yasuhiko Asada, Atsuhiko Ishida, Isamu Kaeshita
Biochim. Biophys. Acta., 1810, 620-629 (2011)

Regulation of TCR Vg2 gene rearrangement by the helix-loop-helix protein, E2A
Masatoshi Nozaki, Kousho Wakae, Nobuyuki Tamaki, Shuji Sakamoto, Kiyouhisa Ohnishi, Toshifumi Uejima, Nagahiro Minato, Itaru Yanagihara, Yasutoshi Agata
Int. Immunol., 23, 297-305 (2011)

A novel and comprehensivemousemodel of human non-alcoholic steatohepatitiswith the full range of dysmetabolic and histological abnormalities induced by gold thioglucose and a high-fat diet
Mitsunari Ogasawara, Akira Hirose, Masafumi Ono, Kosuke Aritake, Yasuko Nozaki, Masaya Takahashi, Nobuto Okamoto, Shuji Sakamoto, Shinji Iwasaki, Taketoshi Asanuma, Taketoshi Taniguchi, Yoshihiro Urade, Saburo Onishi, Toshiji Saibara, Jude A Oben
Liver Int., 31, 542-551 (2011)

Dynamic Changes of microRNAs in the Eye during the Development of Experimental Autoimmune Uveoretinitis
Waka Ishida, Ken Fukuda, Takuma Higuchi, Mina Kajisako, Shuji Sakamoto, Atsuki Fukushima
Invest. Ophthalmol. Vis. Sci., 52, 611-617 (2011)

Calcium/calmodulin-dependent protein kinase IV involvement in the
pathophysiology of glucotoxicity in rat pancreatic β-cells
Yasunori Sugiyama, Koji Murao, Hitomi Imachi, Noriyuki Sueyoshi, Toshihiko Ishida, Isamu Kameshita
Metabolism, 60, 145-153 (2011)

microRNA生合成における負の制御
坂本 修士, 樋口 琢磨, 戸高 寛
THE LUNG perspectives, 19(3), 96-103(2011)

<2010年>
The DNA-binding activity of mouse DNA methyltransferase 1 is regulated by phosphorylation with casein kinase 1δ/ε
Yasunori Sgiyama, Naoya Hatano, Noriyuki Sueyoshi, Isao Suetake, Shoji Tajima, Eiji Kinoshita, Emiko Kinoshita-Kikuta, Tohru Koike, Isamu Kameshita
Biochem. J., 427, 489-497 (2010)

A minimum size homologue of Ca2+/calmodulin-dependent protein kinase IV naturally occurring in zebrafish
Takaki Nimura, Yasunori Sugiyama, Noriyuki Sueyoshi, Yasushi Shigeri, Atsuhiko Ishida, Isamu Kameshita
J. Biochem., 147, 857-865 (2010)

<2009年>
The NF90-NF45 Complex Functions as a Negative Regulator
in the MicroRNA Processing Pathway
Shuji Sakamoto, Kazuma Aoki, Takuma Higuchi, Hiroshi Todaka, Keiko Morisawa, Nobuyuki Tamaki, Etsuro Hatano, Atsuki Fukushima, Taketoshi Taniguchi, Yasutoshi Agata
Mol. Cell. Biol., 29, 3754-3569 (2009)

Systemic overexpression of IFN-gamma and IL-5 exacerbates early phase reaction and conjunctival eosinophilia, respectively, in experimental allergic conjunctivitis
Ishida W, Tsuru E, Tominaga A, Miyazaki J, Higuchi T, Sakamoto S, Fukushima A.

Br. J. Ophthalmol., 93(12), 1680-1685 (2009)

<2007年>
Regulation of T Cell Receptor b gene Rearrengements and Allelic Exclusion by the Helix-Loop-Helix Protein, E47
Agata Y, Tamaki N, Sakamoto S, Ikawa T, Masuda K, Kawamoto H, Murre C.

Immunity, 27, 871-884 (2007)

<2006年>
Type I interferons activate apoptosis in a Jurkat cell variant by caspase-dependent and independent mechanisms
Gamero A, Potla R, Sakamoto S, Baker DP, Abraham R, Larner AC.
Cellular Signalling, 18(8),1299-1308 (2006)

<2004年>
Novel roles of TLR3 tyrosine phosphorylation and PI3 kinase in dsRNA-signaling
Sarker SN, Peter KL, Elico CP, Sakamoto S, Pal S, Sen GC.
Nature Structural & Molecular Biology, 11 (11), 1060-1067 (2004)

Histone Deacetylase Activity is Required to Recruit RNA Polymerase II to the Promoter of Selected Interferon Stimulated Early Response Genes
Sakamoto S, Polta R, Larner AC.
J. Biol. Chem., 279 (39), 40362-40367 (2004)

Identification of a novel conserved motif in the Stat family that is required for tyrosine phosphorylation
Gamero A, Sakamoto S, Montenegro J, Larner AC.
J. Biol. Chem., 279 (13), 12379-12385 (2004)

Cells previously desensitized to type I interferons display different mechanisms on activation of stat-dependent gene expression than naïve cells
Sakamoto S, Qin J, Navarro A, Gamero A, Polta R, Yi T, Zhu W, Baker DP, Feldman G, Larner AC.
J. Biol. Chem., 279 (5), 3245-3253 (2004)

<2002年>
Genetic background determines sensitivity to the inhibitory function of NO on T cell proliferation and the amounts of NO production mediated through IFNγ
Ozaki A, Fukushima A, Fukata K, Sakamoto S, Taniguchi T, Hashiguch M, Ueno H.
Microbiology and Immunology, 46(8), 555-563 (2002)

Inhibition of poly (ADP-ribose) polymerase activity by Bcl-2 in association with the ribosomal protein S3a
Song D, Sakamoto S, Taniguchi T.
Biochemistry, 41(3), 929-934 (2002)

<2001年>
Identification of a phorbol ester-responsive element in the IFNγ receptor 1 chain gene
Sakamoto S, Taniguchi T.
J. Biol. Chem., 276(40), 37237-37241 (2001)

Mechanism for maintenance of dominant T helper 1 immune responses in Lewis rats
Sakamoto S, Fukushima A, Ozaki A, Ueno H, Kamakura M, Taniguchi T.
Microbiology and Immunology, 45(5), 373-381 (2001)

Cloning, sequencing, and expression of the meso-diaminopimelate dehydrogenase gene from Bacillus sphaericus
Sakamoto S, Seki M, Nagata S, Misono H.

Journal of Molecular Catalysis: B:Enzymatic, 12, 85-92 (2001)

<2000年>
Molecular Characterization and Nuclear Localization of Rat timeless-like Gene Product
Sakamoto S, Miyazaki K, Fukui H, Oishi K, Hayasaka N, Okada M, Kamakura M, Taniguchi T, Nagai K, Ishida N.
Biochem. Biophys. Res. Commun., 279, 131-138 (2000)

<1999年>
Identification of Carbonic Anhydrase IV and VI mRNA Expression in Human Pancreas and Salivary Glands
Fujikawa-Adachi K, Nishimori I, Sakamoto S, Morita M, Onishi S, Yonezawa S, Hollingsworth.A M.
Pancreas, 18(4), 329-335 (1999)

Phorbol Ester Induction of IFNg Receptors Leads to Enhanced DR α Gene Expression
Sakamoto S, Nie J, Taniguchi T.

Journal of Immunology, 162, 4381-4384 (1999)

A Binding Protein to the DNase I Hypersensitive Site II in HLA-DR a Gene Was Identified as NF90
Sakamoto S, Morisawa K, Ota K, Nie J, Taniguchi T.
Biochemistry, 38(11), 3355-3361 (1999)

<1998年>
Identification of Oct-1 and automodification domain of poly (ADP-ribose) synthetase
Nie J, Sakamoto S, Song D, Qu Z, Ota K, Taniguchi T.
FEBS Letters, 424, 27-32 (1998)